Coding

Part:BBa_K4759020:Design

Designed by: Mengrui Tao   Group: iGEM23_Jiangnan-China   (2023-10-02)


hemA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 246
    Illegal BglII site found at 524
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 178


Design

Recombinant plasmid pCDFDuet-hemA-hemL is expressed in engineered bacteria

Source

a gene coding for glutamyl-tRNA reductase (GluTR)

References

[1]Sultana, N. Microbial biotransformation of bioactive and clinically useful steroids and some salient features of steroids and biotransformation. Steroids 2018, 136. DOI: 10.1016/j.steroids.2018.01.007.